Paul Janmey, University of Pennsylvania
Aihe: Engineering soft materials for optimal cell function
Aika: Tiistai 25.1.2011 klo 14:15
Paikka: F-rakennuksen auditorio (F239a), Otakaari 3
Many cell types are sensitive to mechanical signals. One striking example is the modulation of cell proliferation, morphology, motility, and protein expression in response to substrate stiffness. Differentiation of stem cells, formation of contractile structures in cardiac myocytes, and extension of processes by neurons all depend on the mechanical properties of the material to which these cells adhere. The range of stiffness over which different primary cell types respond can vary over a wide range and generally reflects the elastic modulus of the tissue from which these cells were isolated. Mechanosensing also depends on the type of adhesion receptor by which the cell binds. The physical mechanisms by which cells detect substrate stiffness are less well characterized than the identity of proteins involved in producing the response that ultimately changes cell morphology, stiffness and function. Simultaneous control of substrate stiffness and adhesive patterns suggest that stiffness sensing occurs on a length scale much larger than single molecular linkages and that the time needed for mechanosensing is on the order of a few seconds.
- Janmey, P. A. and R. T. Miller. 2011. Mechanisms of mechanical signaling in development and disease. J Cell Sci 124:9-18.
- Rasi, K., J. Piuhola, M. Czabanka, R. Sormunen, M. Ilves, H. Leskinen, J. Rysa, R. Kerkela, P. Janmey, R. Heljasvaara, K. Peuhkurinen, O. Vuolteenaho, H. Ruskoaho, P. Vajkoczy, T. Pihlajaniemi, and L. Eklund. 2010. Collagen XV Is Necessary for Modeling of the Extracellular Matrix and Its Deficiency Predisposes to Cardiomyopathy. Circulation Research 107:1241-1252.
- Levental, I., K. R. Levental, E. A. Klein, R. Assoian, R. T. Miller, R. G. Wells, and P. A. Janmey. 2010. A simple indentation device for measuring micrometer-scale tissue stiffness. Journal of Physics-Condensed Matter 22: 194120